Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Molecular Therapy Nucleic Acids

Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high level gene knock-in in HSPCs without compromising cell survival

doi: 10.1016/j.omtn.2025.102495

Figure Lengend Snippet: Figure 5. Coupling of rAAV6 with low amounts of mannose allowed high-level nanoblade-mediated knockin CD34+ cells without cell death induction (A) 8E4, 3E5, or 8E5 mannose sugars were incubated for 4 h at RT with 10E12 vg of rAAV6 vectors encoding for the donor template. 10E9 vg rAAV6 vector per condition were analyzed in dot blot using the ADK1 antibody for rAAV6 that recognizes the intact assembled capsid or conca- navalin A, which detected mannose bound to the capsid. (B) 5E8 vg of rAAV6 conjugated with indicated quantities of mannose were separated on a gel, followed by silver staining to reveal the intact capsids. (C and D) CD34+

Article Snippet: Purity of the selected CD34+ cell fraction was evaluated by fluorescence-activated cell sorting (FACS) analysis (FACSCanto, BD) with APC-conjugated anti-CD34 antibody (Miltenyi Biotech).

Techniques: Knock-In, Incubation, Plasmid Preparation, Dot Blot, Silver Staining

Journal: Journal of vascular research

Article Title: Microvascular endothelial cell responses in vitro and in vivo: modulation by zoledronic acid and paclitaxel?

doi: 10.1159/000313876

Figure Lengend Snippet: Fig. 7. The effect of treatment with zole- dronic acid or paclitaxel on spleen vascu- lature. CD1 nude mice were treated with saline, 100 g/kg i.p. zoledronic acid 20 mg/kg i.v. paclitaxel, alone or in combina- tion. Animals were sacrificed and the spleen isolated and processed for histolog- ical analysis of the vasculature following staining with an antibody to CD34. a Con- trol. b Paclitaxel only. c Zoledronic acid only. d Paclitaxel and zoledronic acid.

Article Snippet: Sections were stained using a rat antibody specific for mouse CD34 (MCA1825CA 1: 50, ABDSerotec, UK) and an IgG2A isotype control, EC and vessel morphology were assessed by an experienced histopathologist (S.S.C.) who was blinded to the treatment groups.

Techniques: Saline, Isolation, Staining

Journal: Neural Regeneration Research

Article Title: A vascular endothelial growth factor–loaded chitosanhyaluronic acid hydrogel scaffold enhances the therapeutic effect of adipose-derived stem cells in the context of stroke

doi: 10.4103/nrr.nrr-d-24-00129

Figure Lengend Snippet: Figure 1 ｜ADSC culture and identity verification, and the effect of VEGF on ADSCs. (A) Representative images of ADSCs on days 3 (i) and 5 (ii) of primary culture, as well as third-passage ADSCs (iii). On day 3, the cells were spindle-shaped, isolated, scattered, and adherent. By day 5, the cells had proliferated rapidly, and the cell colonies had expanded. The morphology of the third-passage cells was uniform, displaying a characteristic fish-like, fibrous, and swirling arrangement. Scale bar: 100 μm. (B) Flow cytometric analysis of CD90, CD29, CD34, and CD45 expression on the cell surface of third-passage ADSCs. (C) CCK-8 analysis of the viability of ADSCs treated with different concentrations of VEGF. Data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, vs. Cont group (one-way analysis of variance with Bonferroni’s post hoc test). (D) Enzyme-linked immunosorbent assay analysis of VEGF, b-FGF, TGF-β, BDNF, and IGF-1 concentration by ADSC cells treated with VEGF (50 ng/mL) or left untreated. Data are expressed as mean ± SEM (n = 12). **P < 0.01, ***P < 0.001, vs. VEGF- group (one-way analysis of variance with Bonferroni’s post hoc test [C] or unpaired t-test [D]). ADSCs: Adipose-derived mesenchymal stem cells; BDNF: brain-derived neurotrophic factor; b-FGF: basic fibroblast growth factor; Cont: control; IGF- 1: insulin-like growth factor-1; TGF-β: transforming growth factor-β; VEGF: vascular endothelial growth factor.

Article Snippet: The following fluorochrome-labeled antibodies were utilized: APC-cy7-CD45 (BD Biosciences, San Jose, CA, USA, Cat# 561586, RRID: AB_10896305), PE-CD34 (RayBiotech, Norcross, GA, USA, Cat# 188-10041-PE, RRID: AB_2315728), FITC-CD90 (BD Biosciences, Cat# 561973, RRID: AB_395588), and BV421-CD29 (BD Biosciences, Cat# 564131, RRID: AB_2738613).

Techniques: Isolation, Expressing, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Derivative Assay, Control